Detailed information of TA system TA180491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6551..7278
• Replicon: plasmid pJX11-1
• Accession: NZ_CP064209
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: IS500_RS25530
• Protein ID: WP_011251285.1
• Coordinates: 6967..7278 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IS500_RS25525
• Protein ID: WP_194797339.1
• Coordinates: 6551..6970 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS500_RS25495	1719..2189	-	471	WP_048333570.1	hypothetical protein	-
IS500_RS25500	2382..3137	-	756	Protein_4	hypothetical protein	-
IS500_RS25505	3733..4176	+	444	WP_048333569.1	hypothetical protein	-
IS500_RS25510	4197..4985	+	789	WP_149999082.1	hypothetical protein	-
IS500_RS25515	4999..5334	+	336	Protein_7	conjugal transfer protein TraI	-
IS500_RS25520	5436..6404	-	969	WP_074428168.1	IS5 family transposase	-
IS500_RS25525	6551..6970	-	420	WP_194797339.1	helix-turn-helix domain-containing protein	Antitoxin
IS500_RS25530	6967..7278	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
IS500_RS25535	7483..7941	-	459	Protein_11	DDE-type integrase/transposase/recombinase	-
IS500_RS25540	8055..8752	+	698	Protein_12	IS1 family transposase	-
IS500_RS25545	8769..9203	+	435	Protein_13	thioredoxin fold domain-containing protein	-
IS500_RS25550	9220..9531	+	312	WP_011251282.1	hypothetical protein	-
IS500_RS25555	9545..9886	+	342	WP_011251281.1	hypothetical protein	-
IS500_RS25560	9946..10902	+	957	WP_011251280.1	DsbA family protein	-
IS500_RS25565	11019..11594	+	576	WP_074424927.1	hypothetical protein	-
IS500_RS25570	11702..12217	+	516	WP_094819131.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..228028	228028
	-	inside	IScluster/Tn	-	-	729..8752	8023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T180491 WP_011251285.1 NZ_CP064209:c7278-6967 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T180491 NZ_CP085482:c91539-91189 [Proteus sp. NMG38-2]
ATGTGGGAAATATTAACGAGAGATCTCTTTGATTCTTGGTTCGAAGAGCAAAATGAGGAAACTCAGATTGAAGTGTTGGC
AGTGTTAATGATTTTACGTGAGGATGGCCCCAATCTTGGACGCCCTCAAGTTGACACCCTGAAGGGCTCACAGTTTCCCA
ACATGAAGGAACTGCGAATTCAGGTTGGTGGTCATCCGATTAGAGCGTGTTTCGCATTTGACCCAATTCGTCGTGGCATT
GTGTTATGTGCCGGTGATAAGAAAGGAAAAGATGAAACCCGTTTCTACAAGAAATTAATCAAGATGGCCGATGCGGAATA
TGCAGCGCACTTATTAGAGCAGGAGAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15362.49 Da
• Isoelectric Point: 4.4420

>AT180491 WP_194797339.1 NZ_CP064209:c6970-6551 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGLRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT180491 NZ_CP085482:c91187-90888 [Proteus sp. NMG38-2]
ATGTCCACTTTAAATGAACTACTTGCAAAACAATCCCCCGAAACGCTGGCAAAAATTGAAGACCGAGCAGAAGAAATCCG
GCGTGAAATCACACTAGCTAAAATTCGTGAGGAACTGAAACTTTCTCAAACTGAGCTAGCTCAATCACTGGGCGTTTCGC
AACCTTCTATTGCTAAGCTTGAAAATGTTGATAACGATCCGAAGCTTTCAACTCTGAAGCGCTACATTAAAGCTTTAGGC
GGTGAACTTTCTATCGATGTAACGTTACCGAATGGTAAGAGAATTGGATTGCATCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1