Detailed information of TA system TA180474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 195194..195921
• Replicon: plasmid pJX12-1
• Accession: NZ_CP064206
• Organism: Klebsiella pneumoniae strain JX-CR-hvKP-12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: IS504_RS27350
• Protein ID: WP_011251285.1
• Coordinates: 195194..195505 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IS504_RS27355
• Protein ID: WP_011251286.1
• Coordinates: 195502..195921 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS504_RS27310	190255..190770	-	516	WP_041937820.1	hypothetical protein	-
IS504_RS27315	190878..191453	-	576	WP_074424927.1	hypothetical protein	-
IS504_RS27320	191570..192526	-	957	WP_011251280.1	DsbA family protein	-
IS504_RS27325	192586..192927	-	342	WP_011251281.1	hypothetical protein	-
IS504_RS27330	192941..193252	-	312	WP_011251282.1	hypothetical protein	-
IS504_RS27335	193269..193703	-	435	Protein_209	thioredoxin fold domain-containing protein	-
IS504_RS27340	193720..194417	-	698	Protein_210	IS1 family transposase	-
IS504_RS27345	194531..194989	+	459	Protein_211	DDE-type integrase/transposase/recombinase	-
IS504_RS27350	195194..195505	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
IS504_RS27355	195502..195921	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
IS504_RS27360	196068..197036	+	969	WP_074428168.1	IS5 family transposase	-
IS504_RS27365	197138..197473	-	336	Protein_215	conjugal transfer protein TraI	-
IS504_RS27370	197487..198275	-	789	WP_040217257.1	hypothetical protein	-
IS504_RS27375	198296..198739	-	444	WP_048333569.1	hypothetical protein	-
IS504_RS27380	199335..200090	+	756	Protein_218	hypothetical protein	-
IS504_RS27385	200283..200753	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..230835	230835
	-	inside	IScluster/Tn	-	-	193720..206042	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T180474 WP_011251285.1 NZ_CP064206:195194-195505 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T180474 NZ_CP085469:2435-2698 [Aeromonas caviae]
TTGGCTTGGAAAGTTGAACTGACCGATACCGCTGTTAAGCAGTTGGCTAGGCTCGATAAAACCCAAGCCCAGCGGATCAC
CAAGTACCTACGGCGGGTGATGATGCTGGAAGACCCAAGGGATGCAGGTAAAGCATTAACCGGCAATCTACGTACCTACT
GGCGTTATCGAGTGGGGGATTATCGGGTTATCTGTGAGATCAGGGATGCCGAGCTGGTGATCGTGGCCGTTGAGATCGGA
CATCGTAGTGAGGTGTATGGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT180474 WP_011251286.1 NZ_CP064206:195502-195921 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT180474 NZ_CP085469:2227-2451 [Aeromonas caviae]
ATGTTAGCGATACGCTTGCCTTCGGAGATCGAGGAGCGTTTGGCTCATTTGGCGCAAGAAACAGGGAGAACCAAGACCTA
CTATGCGCGTGAGGCTATCTTGACGCACTTGGAAGACCTAGAGGATTACTATCTGGCTGAGCGGGTGATGGAGAAGGTGC
GCAAGGGTGAAGAGCGCCTGTTCTCACTAGATGACGTGGAGCGTGACCTTGGCTTGGAAAGTTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1