Detailed information of TA system TA180428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2307757..2308319
• Replicon: chromosome
• Accession: NZ_CP064180
• Organism: Citrobacter amalonaticus strain CA71

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6Z3F2
• Locus tag: ISX58_RS10910
• Protein ID: WP_044257939.1
• Coordinates: 2307757..2308035 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISX58_RS10915
• Protein ID: WP_043000693.1
• Coordinates: 2308035..2308319 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISX58_RS10890	2302882..2303313	-	432	WP_043000697.1	peroxiredoxin OsmC	-
ISX58_RS10895	2303541..2303684	+	144	WP_042319017.1	stationary-phase-induced ribosome-associated protein	-
ISX58_RS10900	2303856..2305553	+	1698	WP_044257943.1	malate dehydrogenase	-
ISX58_RS10905	2305862..2307577	+	1716	WP_044257940.1	ATP-binding cassette domain-containing protein	-
ISX58_RS10910	2307757..2308035	+	279	WP_044257939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISX58_RS10915	2308035..2308319	+	285	WP_043000693.1	HigA family addiction module antidote protein	Antitoxin
ISX58_RS10920	2308364..2308966	-	603	WP_044257938.1	inorganic diphosphatase	-
ISX58_RS10925	2309093..2309749	-	657	WP_043000691.1	formate dehydrogenase-N subunit gamma	-
ISX58_RS10930	2309742..2310626	-	885	WP_043000690.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10525.03 Da
• Isoelectric Point: 8.1206

>T180428 WP_044257939.1 NZ_CP064180:2307757-2308035 [Citrobacter amalonaticus]
MIMNFRHKGLRDLFLHGKTSGVLAKHVKRLRHRLAVIDAAGCVNDINMPGYRLHPLSGDRDGVWSISVSGNWRMTFEFVN
GDAYILDDEDYH

Nucleotide

• Length: 279 bp

>T180428 NZ_CP085296:22732-23073 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10370.07 Da
• Isoelectric Point: 10.2982

>AT180428 WP_043000693.1 NZ_CP064180:2308035-2308319 [Citrobacter amalonaticus]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAQAQ
KSVDVSRLRRLAVQ

Nucleotide

• Length: 285 bp

>AT180428 NZ_CP085296:22502-22732 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Structures

Toxin

• AlphaFold DB: A0A7L6Z3F2