Detailed information of TA system TA180397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3793385..3794184
• Replicon: chromosome
• Accession: NZ_CP064167
• Organism: Escherichia coli O157:H7 strain Wll001

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: IMX21_RS18610
• Protein ID: WP_000347273.1
• Coordinates: 3793385..3793849 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: IMX21_RS18615
• Protein ID: WP_001302819.1
• Coordinates: 3793849..3794184 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMX21_RS18580	3788722..3788820	-	99	Protein_3623	PTS N-acetylgalactosamine transporter subunit IIA	-
IMX21_RS18585	3788838..3789716	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
IMX21_RS18590	3789706..3790485	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
IMX21_RS18595	3790496..3790969	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
IMX21_RS18600	3790992..3792272	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
IMX21_RS18605	3792521..3793330	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
IMX21_RS18610	3793385..3793849	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
IMX21_RS18615	3793849..3794184	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
IMX21_RS18620	3794333..3795904	-	1572	WP_001273776.1	galactarate dehydratase	-
IMX21_RS18625	3796279..3797613	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
IMX21_RS18630	3797629..3798399	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T180397 WP_000347273.1 NZ_CP064167:c3793849-3793385 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T180397 NZ_CP085283:507455-507805 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT180397 WP_001302819.1 NZ_CP064167:c3794184-3793849 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT180397 NZ_CP085283:507168-507449 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: V0SSH7