Detailed information of TA system TA180386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2029550..2030244
• Replicon: chromosome
• Accession: NZ_CP064167
• Organism: Escherichia coli O157:H7 strain Wll001

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: IMX21_RS10290
• Protein ID: WP_001263495.1
• Coordinates: 2029550..2029948 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IMX21_RS10295
• Protein ID: WP_000554757.1
• Coordinates: 2029951..2030244 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	2025137..2025217	-	81	NuclAT_12	-	-
-	2025137..2025217	-	81	NuclAT_12	-	-
-	2025137..2025217	-	81	NuclAT_12	-	-
-	2025137..2025217	-	81	NuclAT_12	-	-
IMX21_RS10265	2025813..2026271	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IMX21_RS10270	2026532..2027989	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
IMX21_RS10275	2028046..2028567	-	522	Protein_2014	peptide chain release factor H	-
IMX21_RS10280	2028566..2028769	-	204	Protein_2015	RNA ligase RtcB family protein	-
IMX21_RS10285	2029088..2029540	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
IMX21_RS10290	2029550..2029948	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IMX21_RS10295	2029951..2030244	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IMX21_RS10300	2030296..2031351	-	1056	WP_001226188.1	DNA polymerase IV	-
IMX21_RS10305	2031422..2032207	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
IMX21_RS10310	2032152..2033891	+	1740	WP_009625779.1	flagellar biosynthesis protein FlhA	-
IMX21_RS10315	2034036..2034533	-	498	Protein_2022	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1992709..2035482	42773

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T180386 WP_001263495.1 NZ_CP064167:c2029948-2029550 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T180386 NZ_CP085202:c4316512-4316237 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT180386 WP_000554757.1 NZ_CP064167:c2030244-2029951 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT180386 NZ_CP085202:c4316240-4315881 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6