Detailed information of TA system TA180369

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 363959..364330
• Replicon: chromosome
• Accession: NZ_CP064167
• Organism: Escherichia coli O157:H7 strain Wll001

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: IMX21_RS02020
• Protein ID: WP_001443846.1
• Coordinates: 364181..364330 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	363959..364137 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMX21_RS01990	359711..359881	+	171	WP_001625136.1	hypothetical protein	-
IMX21_RS01995	359914..361287	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
IMX21_RS02000	361416..362351	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
IMX21_RS02005	362403..363638	-	1236	WP_000040839.1	site-specific integrase	-
IMX21_RS02010	363640..363855	-	216	WP_000079604.1	excisionase XisR	-
IMX21_RS02015	363955..364143	-	189	WP_001302840.1	DUF1187 family protein	-
-	363959..364137	+	179	NuclAT_0	-	Antitoxin
-	363959..364137	+	179	NuclAT_0	-	Antitoxin
-	363959..364137	+	179	NuclAT_0	-	Antitoxin
-	363959..364137	+	179	NuclAT_0	-	Antitoxin
IMX21_RS02020	364181..364330	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
IMX21_RS02025	364386..365195	-	810	WP_000166313.1	recombination protein RecT	-
IMX21_RS02030	365188..367788	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
IMX21_RS02035	367890..368165	-	276	WP_001344816.1	hypothetical protein	-
IMX21_RS02040	368240..368410	-	171	WP_001352098.1	conserved protein, Rac prophage	-
IMX21_RS02045	368410..368631	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	342281..482826	140545

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T180369 WP_001443846.1 NZ_CP064167:c364330-364181 [Escherichia coli O157:H7]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T180369 NZ_CP085197:c3267871-3266549 [Klebsiella quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGCGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGAGGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATTACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGAGCGGATCGTCAGGCGCTATCAG
GCGCGTTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGGCGGGACAGCGTAGGGGCGGTGACTCTGCTGCCGCCGGG
GGAAGAGGCGCATCTTGCCGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAGCTGACCGCTTTGCTGACCGCCTACCAGT
CGGATATTCCTCTCGGGATGGTGACCGGCCAGGACGATTTTCGGATCTCGGTGGCCGGCGCTCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCTTGGCGGTGCCGGAGGCGGAGATTATCAACACGCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATACCGTCGTCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAGGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGCGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACGGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTCCCCCGGGAGCAGATGCTGGCGATCCTGGGCGAGTTTGCCGACCGCGTGCCG
CAGGCCATCGAAAGCGCCCGCCGGACGCTGCCGTCGGACTTTTCCGCCCACGTCTGGCGGGCCATCACGGAGAACATGCT
TAAACTGCACGCCCGCCTGCAGCAAGGGCTGCTGGCGGGATAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT180369 NZ_CP064167:363959-364137 [Escherichia coli O157:H7]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin