Detailed information of TA system TA180159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 36973..37619
• Replicon: plasmid unnamed2
• Accession: NZ_CP064105
• Organism: Escherichia coli strain IR53017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ISU84_RS26665
• Protein ID: WP_000269912.1
• Coordinates: 36973..37320 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISU84_RS26670
• Protein ID: WP_001673379.1
• Coordinates: 37320..37619 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU84_RS26640	32922..33539	+	618	WP_001523080.1	tail fiber assembly protein	-
ISU84_RS26645	33503..34048	-	546	WP_001704996.1	transferase	-
ISU84_RS26650	34674..35510	+	837	WP_024171747.1	replication initiator protein RepA	-
ISU84_RS26655	36154..36441	+	288	WP_000356589.1	hypothetical protein	-
ISU84_RS26660	36465..36728	+	264	WP_000424604.1	hypothetical protein	-
ISU84_RS26665	36973..37320	+	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISU84_RS26670	37320..37619	+	300	WP_001673379.1	XRE family transcriptional regulator	Antitoxin
ISU84_RS26675	37653..37937	-	285	WP_001673380.1	hypothetical protein	-
ISU84_RS26680	38205..38585	+	381	WP_000061763.1	hypothetical protein	-
ISU84_RS26685	38649..38921	+	273	WP_089591621.1	helix-turn-helix domain-containing protein	-
ISU84_RS26690	39528..40709	-	1182	WP_171874464.1	ORF6N domain-containing protein	-
ISU84_RS26695	40706..40897	-	192	WP_000183351.1	hypothetical protein	-
ISU84_RS26700	42202..42450	-	249	WP_000730008.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..47492	47492

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T180159 WP_000269912.1 NZ_CP064105:36973-37320 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T180159 NZ_CP085074:c4226096-4225809 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11069.90 Da
• Isoelectric Point: 5.8899

>AT180159 WP_001673379.1 NZ_CP064105:37320-37619 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFHV

Nucleotide

• Length: 300 bp

>AT180159 NZ_CP085074:c4225822-4225520 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA