Detailed information of TA system TA180153

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51197..51623
• Replicon: plasmid unnamed1
• Accession: NZ_CP064104
• Organism: Escherichia coli strain IR53017

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ISU84_RS25855
• Protein ID: WP_001312861.1
• Coordinates: 51197..51355 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	51399..51623 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU84_RS25830	46571..47260	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
ISU84_RS25835	47447..47830	-	384	WP_001151524.1	relaxosome protein TraM	-
ISU84_RS25840	48151..48753	+	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
ISU84_RS25845	49050..49871	-	822	WP_001234426.1	DUF945 domain-containing protein	-
ISU84_RS25850	49989..50276	-	288	WP_000107535.1	hypothetical protein	-
ISU84_RS25855	51197..51355	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	51399..51623	-	225	NuclAT_0	-	Antitoxin
-	51399..51623	-	225	NuclAT_0	-	Antitoxin
-	51399..51623	-	225	NuclAT_0	-	Antitoxin
-	51399..51623	-	225	NuclAT_0	-	Antitoxin
ISU84_RS25860	51435..51623	+	189	WP_001299721.1	hypothetical protein	-
ISU84_RS25865	51635..52354	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
ISU84_RS25870	52351..52785	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
ISU84_RS25875	52840..53037	-	198	Protein_66	hypothetical protein	-
ISU84_RS25880	53065..53298	-	234	WP_000005990.1	DUF905 family protein	-
ISU84_RS25885	53366..53905	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
ISU84_RS25890	53931..54137	-	207	WP_000275856.1	hypothetical protein	-
ISU84_RS25895	54547..54759	-	213	WP_001348622.1	hypothetical protein	-
ISU84_RS25900	54785..55350	-	566	Protein_71	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aadA5 / qacE / sul1 / mph(A) / tet(B)	iutA / iucD / iucC / iucB / iucA	1..145347	145347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T180153 WP_001312861.1 NZ_CP064104:c51355-51197 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T180153 NZ_CP085074:c2600187-2600011 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT180153 NZ_CP064104:c51623-51399 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin