Detailed information of TA system TA180149

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 11130..11384
• Replicon: plasmid unnamed1
• Accession: NZ_CP064104
• Organism: Escherichia coli strain IR53017

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: ISU84_RS25625
• Protein ID: WP_015387399.1
• Coordinates: 11130..11336 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	11323..11384 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU84_RS25595	6377..7288	-	912	WP_000440183.1	carbamate kinase	-
ISU84_RS25600	7299..8519	-	1221	WP_000410951.1	arginine deiminase	-
ISU84_RS25605	9412..9894	+	483	Protein_12	VENN motif pre-toxin domain-containing protein	-
ISU84_RS25610	9840..10286	-	447	Protein_13	incFII family plasmid replication initiator RepA	-
ISU84_RS25615	10279..10353	-	75	WP_032336874.1	RepA leader peptide Tap	-
ISU84_RS25620	10589..10846	-	258	WP_000083833.1	replication regulatory protein RepA	-
ISU84_RS25625	11130..11336	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	11323..11384	+	62	NuclAT_1	-	Antitoxin
-	11323..11384	+	62	NuclAT_1	-	Antitoxin
-	11323..11384	+	62	NuclAT_1	-	Antitoxin
-	11323..11384	+	62	NuclAT_1	-	Antitoxin
ISU84_RS25630	11523..11705	-	183	WP_000968309.1	hypothetical protein	-
ISU84_RS25635	11806..12422	+	617	Protein_18	IS1 family transposase	-
ISU84_RS25640	12460..14031	-	1572	WP_113305802.1	IS66 family transposase	-
ISU84_RS25645	14051..14398	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
ISU84_RS25650	14398..15075	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
ISU84_RS25655	15130..15219	+	90	Protein_22	IS1 family transposase	-
ISU84_RS25660	15520..15732	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aadA5 / qacE / sul1 / mph(A) / tet(B)	iutA / iucD / iucC / iucB / iucA	1..145347	145347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T180149 WP_015387399.1 NZ_CP064104:c11336-11130 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T180149 NZ_CP085074:1954388-1954642 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT180149 NZ_CP064104:11323-11384 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin