Detailed information of TA system TA180148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5064578..5065180
• Replicon: chromosome
• Accession: NZ_CP064103
• Organism: Escherichia coli strain IR53017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ISU84_RS24495
• Protein ID: WP_000897305.1
• Coordinates: 5064869..5065180 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISU84_RS24490
• Protein ID: WP_000356397.1
• Coordinates: 5064578..5064868 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU84_RS24470	5061080..5061982	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
ISU84_RS24475	5061979..5062614	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ISU84_RS24480	5062611..5063540	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
ISU84_RS24485	5063755..5063973	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
ISU84_RS24490	5064578..5064868	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ISU84_RS24495	5064869..5065180	-	312	WP_000897305.1	hypothetical protein	Toxin
ISU84_RS24500	5065409..5066317	+	909	WP_001318161.1	alpha/beta hydrolase	-
ISU84_RS24505	5066381..5067322	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ISU84_RS24510	5067367..5067804	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ISU84_RS24515	5067801..5068673	-	873	WP_000920762.1	virulence factor BrkB family protein	-
ISU84_RS24520	5068667..5069266	-	600	WP_001314324.1	glucose-1-phosphatase	-
ISU84_RS24525	5069365..5070150	-	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T180148 WP_000897305.1 NZ_CP064103:c5065180-5064869 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T180148 NZ_CP085074:1954140-1954391 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT180148 WP_000356397.1 NZ_CP064103:c5064868-5064578 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT180148 NZ_CP085074:1954388-1954642 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7