Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4083241..4083935 Replicon chromosome
Accession NZ_CP064103
Organism Escherichia coli strain IR53017

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag ISU84_RS19665 Protein ID WP_001263491.1
Coordinates 4083241..4083639 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag ISU84_RS19670 Protein ID WP_000554755.1
Coordinates 4083642..4083935 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU84_RS19635 4078503..4079960 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
ISU84_RS19640 4079969..4080250 + 282 WP_022645226.1 hypothetical protein -
ISU84_RS19645 4080267..4080776 - 510 WP_001361775.1 hydrolase -
ISU84_RS19650 4080838..4081452 - 615 WP_022645225.1 peptide chain release factor H -
ISU84_RS19655 4081449..4082588 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
ISU84_RS19660 4082779..4083231 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
ISU84_RS19665 4083241..4083639 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ISU84_RS19670 4083642..4083935 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ISU84_RS19675 4083987..4085042 - 1056 WP_022645222.1 DNA polymerase IV -
ISU84_RS19680 4085113..4085898 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
ISU84_RS19685 4085870..4087582 + 1713 Protein_3863 flagellar biosynthesis protein FlhA -
ISU84_RS19690 4087680..4088453 - 774 WP_022645219.1 C40 family peptidase -
ISU84_RS19695 4088639..4088899 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T180142 WP_001263491.1 NZ_CP064103:c4083639-4083241 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T180142 NZ_CP085074:c652467-652003 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT180142 WP_000554755.1 NZ_CP064103:c4083935-4083642 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT180142 NZ_CP085074:c652802-652467 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References