Detailed information of TA system TA180124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 636923..637722
• Replicon: chromosome
• Accession: NZ_CP064103
• Organism: Escherichia coli strain IR53017

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: ISU84_RS03025
• Protein ID: WP_000347273.1
• Coordinates: 636923..637387 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: ISU84_RS03030
• Protein ID: WP_001308975.1
• Coordinates: 637387..637722 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU84_RS02995	631924..632358	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
ISU84_RS03000	632376..633254	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ISU84_RS03005	633244..634023	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ISU84_RS03010	634034..634507	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ISU84_RS03015	634530..635810	-	1281	WP_206356523.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ISU84_RS03020	636059..636868	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
ISU84_RS03025	636923..637387	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ISU84_RS03030	637387..637722	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ISU84_RS03035	637871..639442	-	1572	WP_023908830.1	galactarate dehydratase	-
ISU84_RS03040	639817..641151	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
ISU84_RS03045	641167..641937	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T180124 WP_000347273.1 NZ_CP064103:c637387-636923 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T180124 NZ_CP085069:2096074-2096177 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT180124 WP_001308975.1 NZ_CP064103:c637722-637387 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT180124 NZ_CP085069:c2096215-2095944 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08