Detailed information of TA system TA180099

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 960771..961096
• Replicon: chromosome
• Accession: NZ_CP064096
• Organism: Bacillus subtilis strain N1142-3at

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: P54373
• Locus tag: IRJ28_RS05025
• Protein ID: WP_004398662.1
• Coordinates: 960917..961096 (-)
• Length: 60 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	960771..960993 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRJ28_RS04980 (956140)	956140..956451	+	312	WP_003229923.1	YqbF domain-containing protein	-
IRJ28_RS04985 (956455)	956455..956850	+	396	WP_004398566.1	DUF3199 family protein	-
IRJ28_RS04990 (956847)	956847..957209	+	363	WP_003229925.1	YqbH/XkdH family protein	-
IRJ28_RS04995 (957206)	957206..957709	+	504	WP_003246050.1	HK97 gp10 family phage protein	-
IRJ28_RS05000 (957722)	957722..958159	+	438	WP_003229927.1	hypothetical protein	-
IRJ28_RS05005 (958156)	958156..958347	+	192	WP_010886574.1	hypothetical protein	-
IRJ28_RS05010 (958348)	958348..959748	+	1401	WP_003229929.1	phage tail sheath family protein	-
IRJ28_RS05015 (959751)	959751..960194	+	444	WP_003229930.1	phage tail tube protein	-
IRJ28_RS05020 (960448)	960448..960537	-	90	WP_075058862.1	type I toxin-antitoxin system toxin BsrH	-
- (960771)	960771..960993	+	223	NuclAT_0	-	Antitoxin
- (960771)	960771..960993	+	223	NuclAT_0	-	Antitoxin
- (960771)	960771..960993	+	223	NuclAT_0	-	Antitoxin
- (960771)	960771..960993	+	223	NuclAT_0	-	Antitoxin
IRJ28_RS05025 (960917)	960917..961096	-	180	WP_004398662.1	type I toxin-antitoxin system toxin TxpA	Toxin
IRJ28_RS05030 (961242)	961242..961691	+	450	WP_003229933.1	phage portal protein	-
IRJ28_RS05035 (961733)	961733..961870	+	138	WP_003229934.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	916691..1062127	145436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6739.19 Da
• Isoelectric Point: 8.0370

>T180099 WP_004398662.1 NZ_CP064096:c961096-960917 [Bacillus subtilis]
MSTYESLMVMIGFANLIGGIMTWVISLLTLLFMLRKKDTHPIYITVKEKCLHEDPPIKG

Nucleotide

• Length: 180 bp

>T180099 NZ_CP085063:3537043-3537150 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Nucleotide

• Length: 223 bp

>AT180099 NZ_CP064096:960771-960993 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC

Structures

Toxin

• AlphaFold DB: A0A164X333

Antitoxin