Detailed information of TA system TA180023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3966127..3966791
• Replicon: chromosome
• Accession: NZ_CP064065
• Organism: Rhodococcus sp. M8

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: ISO16_RS17940
• Protein ID: WP_072633872.1
• Coordinates: 3966127..3966480 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: ISO16_RS17945
• Protein ID: WP_072633873.1
• Coordinates: 3966486..3966791 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISO16_RS17920	3962750..3962941	-	192	Protein_3539	RraA family protein	-
ISO16_RS17925	3962939..3963016	-	78	Protein_3540	filamentation induced by cAMP protein fic	-
ISO16_RS17930	3963230..3964705	-	1476	WP_072633870.1	glyceraldehyde-3-phosphate dehydrogenase	-
ISO16_RS17935	3965236..3965561	+	326	Protein_3542	DUF4192 family protein	-
ISO16_RS17940	3966127..3966480	+	354	WP_072633872.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISO16_RS17945	3966486..3966791	+	306	WP_072633873.1	XRE family transcriptional regulator	Antitoxin
ISO16_RS17950	3967310..3969019	+	1710	Protein_3545	acyl-CoA dehydrogenase	-
ISO16_RS17955	3969109..3969780	-	672	Protein_3546	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (30-92)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13670.45 Da
• Isoelectric Point: 5.2636

>T180023 WP_072633872.1 NZ_CP064065:3966127-3966480 [Rhodococcus sp. M8]
MTWDVDLEYIEDWLIDLDQDTYEQVVAAIELLAERGPQLGRPLVDTVKSSRHKNMKELRPGSSGRSELRVLFAFDPQRRA
IFLVAGDKAGKWDKWYRVNIPRADDRFDDHLNRLEGK

Nucleotide

• Length: 354 bp

>T180023 NZ_CP084988:61065-61370 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11691.45 Da
• Isoelectric Point: 10.4345

>AT180023 WP_072633873.1 NZ_CP064065:3966486-3966791 [Rhodococcus sp. M8]
MGISLEDLLAKRPVRRNAVEAHKARMLDQVRAYRLKELRKAQHLTQKQVAERLEVGQNRVSTIERGELDRIQLDTLRRYV
DAVGGTLRIEVELGDDRFQIA

Nucleotide

• Length: 306 bp

>AT180023 NZ_CP084988:60845-61063 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA