Detailed information of TA system TA179915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4915331..4915933
• Replicon: chromosome
• Accession: NZ_CP063983
• Organism: Escherichia coli strain 25DN

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: IR210_RS23470
• Protein ID: WP_000897302.1
• Coordinates: 4915622..4915933 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IR210_RS23465
• Protein ID: WP_000356397.1
• Coordinates: 4915331..4915621 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IR210_RS23440	4911405..4912306	+	902	Protein_4586	formate dehydrogenase O subunit beta	-
IR210_RS23445	4912303..4912938	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IR210_RS23450	4912935..4913864	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
IR210_RS23455	4914080..4914298	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
IR210_RS23460	4914694..4914972	-	279	WP_001296612.1	hypothetical protein	-
IR210_RS23465	4915331..4915621	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
IR210_RS23470	4915622..4915933	-	312	WP_000897302.1	hypothetical protein	Toxin
IR210_RS23475	4916163..4917071	+	909	WP_001305059.1	alpha/beta hydrolase	-
IR210_RS23480	4917135..4918076	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IR210_RS23485	4918121..4918558	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IR210_RS23490	4918555..4919427	-	873	WP_000920762.1	virulence factor BrkB family protein	-
IR210_RS23495	4919421..4920020	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T179915 WP_000897302.1 NZ_CP063983:c4915933-4915622 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T179915 NZ_CP084899:c479532-479314 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT179915 WP_000356397.1 NZ_CP063983:c4915621-4915331 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT179915 NZ_CP084899:c479932-479558 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: S1P416