Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1919362..1919864 Replicon chromosome
Accession NZ_CP063983
Organism Escherichia coli strain 25DN

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag IR210_RS08975 Protein ID WP_000767822.1
Coordinates 1919610..1919864 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IR210_RS08970 Protein ID WP_001259255.1
Coordinates 1919362..1919613 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IR210_RS08945 1914540..1915607 - 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IR210_RS08950 1915607..1916677 - 1071 WP_000108995.1 histidinol-phosphate transaminase -
IR210_RS08955 1916674..1917978 - 1305 WP_001297912.1 histidinol dehydrogenase -
IR210_RS08960 1917984..1918883 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
IR210_RS08965 1919029..1919079 - 51 WP_001364200.1 his operon leader peptide -
IR210_RS08970 1919362..1919613 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IR210_RS08975 1919610..1919864 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IR210_RS08980 1919947..1920771 + 825 WP_000754733.1 SDR family oxidoreductase -
IR210_RS08985 1920817..1921746 + 930 Protein_1758 LysR family transcriptional regulator -
IR210_RS08990 1921961..1922023 + 63 WP_010723108.1 membrane protein YoeI -
IR210_RS08995 1922013..1923371 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T179892 WP_000767822.1 NZ_CP063983:1919610-1919864 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T179892 NZ_CP084898:c1414805-1414611 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT179892 WP_001259255.1 NZ_CP063983:1919362-1919613 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT179892 NZ_CP084898:1414434-1414612 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References