Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1213115..1213842 Replicon chromosome
Accession NZ_CP063983
Organism Escherichia coli strain 25DN

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag IR210_RS05785 Protein ID WP_000547555.1
Coordinates 1213115..1213426 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag IR210_RS05790 Protein ID WP_000126293.1
Coordinates 1213423..1213842 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IR210_RS05755 1208256..1209965 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
IR210_RS05760 1209975..1210517 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
IR210_RS05765 1210517..1211284 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
IR210_RS05770 1211281..1211691 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
IR210_RS05775 1211684..1212154 + 471 WP_194446282.1 hydrogenase maturation peptidase HycI -
IR210_RS05780 1212197..1212952 + 756 WP_001305268.1 hypothetical protein -
IR210_RS05785 1213115..1213426 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
IR210_RS05790 1213423..1213842 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
IR210_RS05795 1213934..1214362 - 429 WP_000536064.1 hypothetical protein -
IR210_RS05800 1214747..1215274 + 528 WP_001078777.1 electron transport protein HydN -
IR210_RS05805 1215427..1217676 + 2250 Protein_1141 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T179891 WP_000547555.1 NZ_CP063983:1213115-1213426 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T179891 NZ_CP084898:c1271086-1270979 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT179891 WP_000126293.1 NZ_CP063983:1213423-1213842 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT179891 NZ_CP084898:1271133-1271199 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References