Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 621468..622267 Replicon chromosome
Accession NZ_CP063983
Organism Escherichia coli strain 25DN

Toxin (Protein)


Gene name yhaV Uniprot ID Q8FDB4
Locus tag IR210_RS03020 Protein ID WP_000347252.1
Coordinates 621468..621932 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag IR210_RS03025 Protein ID WP_001296435.1
Coordinates 621932..622267 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IR210_RS02990 616469..616903 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
IR210_RS02995 616921..617799 - 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IR210_RS03000 617789..618568 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IR210_RS03005 618579..619052 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IR210_RS03010 619075..620355 - 1281 WP_194446222.1 tagatose-bisphosphate aldolase subunit KbaZ -
IR210_RS03015 620604..621413 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IR210_RS03020 621468..621932 - 465 WP_000347252.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IR210_RS03025 621932..622267 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IR210_RS03030 622416..623987 - 1572 WP_001273940.1 galactarate dehydratase -
IR210_RS03035 624362..625696 + 1335 WP_194446223.1 galactarate/glucarate/glycerate transporter GarP -
IR210_RS03040 625712..626482 + 771 WP_001058223.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17750.11 Da        Isoelectric Point: 9.4947

>T179888 WP_000347252.1 NZ_CP063983:c621932-621468 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Download         Length: 465 bp

>T179888 NZ_CP084898:c479532-479314 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT179888 WP_001296435.1 NZ_CP063983:c622267-621932 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT179888 NZ_CP084898:c479932-479558 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q8FDB4

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References