Detailed information of TA system TA179886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2679923..2680535
• Replicon: chromosome
• Accession: NZ_CP063982
• Organism: Orrella daihaiensis strain f23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DHf2319_RS12380
• Protein ID: WP_243478665.1
• Coordinates: 2679923..2680261 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DHf2319_RS12385
• Protein ID: WP_243480104.1
• Coordinates: 2680281..2680535 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DHf2319_RS12350 (DHf2319_12615)	2676101..2676805	+	705	WP_243478659.1	NYN domain-containing protein	-
DHf2319_RS12355 (DHf2319_12620)	2676901..2677143	+	243	WP_243478660.1	PbsX family transcriptional regulator	-
DHf2319_RS12360 (DHf2319_12625)	2677143..2677484	+	342	WP_243478661.1	endoribonuclease MazF	-
DHf2319_RS12365 (DHf2319_12630)	2677863..2678006	-	144	WP_243478662.1	hypothetical protein	-
DHf2319_RS12370 (DHf2319_12635)	2678023..2678679	+	657	WP_243478663.1	NYN domain-containing protein	-
DHf2319_RS12375 (DHf2319_12640)	2678710..2679207	+	498	WP_243478664.1	NYN domain-containing protein	-
DHf2319_RS12380 (DHf2319_12645)	2679923..2680261	+	339	WP_243478665.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DHf2319_RS12385 (DHf2319_12650)	2680281..2680535	+	255	WP_243480104.1	helix-turn-helix transcriptional regulator	Antitoxin
DHf2319_RS12390 (DHf2319_12655)	2680768..2681385	+	618	WP_243478666.1	LysE family translocator	-
DHf2319_RS12395 (DHf2319_12660)	2681555..2681809	+	255	WP_243478667.1	DUF2061 domain-containing protein	-
DHf2319_RS12400 (DHf2319_12665)	2681996..2683093	+	1098	WP_243478668.1	patatin-like phospholipase family protein	-
DHf2319_RS12405 (DHf2319_12670)	2683265..2684470	+	1206	WP_243478669.1	porin	-
DHf2319_RS12410 (DHf2319_12675)	2684576..2685511	-	936	WP_243478670.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (17-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-80)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13034.33 Da
• Isoelectric Point: 10.3662

>T179886 WP_243478665.1 NZ_CP063982:2679923-2680261 [Orrella daihaiensis]
MSDQYQIEYYSDSVQMDILNLPDKLAARYIFLTRRMLAIGPNLGEPHTKAIKDGLFELRLKGSEGIARIFYCTLVGKRIV
MLHSFIKKTGRIPSRELETAMNRLKERKREKT

Nucleotide

• Length: 339 bp

>T179886 NZ_CP084898:252301-252699 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9267.66 Da
• Isoelectric Point: 10.4459

>AT179886 WP_243480104.1 NZ_CP063982:2680281-2680535 [Orrella daihaiensis]
MSRQGVKAELERIEHEEGELLDALLKARQDAGLTQAELAKRMGTQAPAVARLERSLATGRHSPSLATLRRYLKACGRSLE
LRVS

Nucleotide

• Length: 255 bp

>AT179886 NZ_CP084898:252005-252298 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA