Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 787158..787755 Replicon chromosome
Accession NZ_CP063954
Organism Klebsiella variicola strain S129-1

Toxin (Protein)


Gene name higB Uniprot ID A0A1F2LYJ8
Locus tag IOD50_RS03685 Protein ID WP_023322191.1
Coordinates 787158..787475 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6H0BIE0
Locus tag IOD50_RS03690 Protein ID WP_023322192.1
Coordinates 787468..787755 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD50_RS03670 782865..784292 - 1428 WP_131010146.1 MFS transporter -
IOD50_RS03675 784401..785267 + 867 WP_022065427.1 helix-turn-helix transcriptional regulator -
IOD50_RS03680 785930..786973 + 1044 WP_016160437.1 DUF2157 domain-containing protein -
IOD50_RS03685 787158..787475 + 318 WP_023322191.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IOD50_RS03690 787468..787755 + 288 WP_023322192.1 helix-turn-helix transcriptional regulator Antitoxin
IOD50_RS03695 788191..788844 - 654 WP_012968755.1 oxygen-insensitive NAD(P)H nitroreductase -
IOD50_RS03700 788964..789332 - 369 WP_008805536.1 MmcQ/YjbR family DNA-binding protein -
IOD50_RS03705 789332..789913 - 582 WP_008805537.1 TetR/AcrR family transcriptional regulator -
IOD50_RS03710 790093..791208 + 1116 WP_194394748.1 MBL fold metallo-hydrolase -
IOD50_RS03715 791239..791580 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
IOD50_RS03720 791597..791845 - 249 WP_008805539.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-89)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T179829 WP_023322191.1 NZ_CP063954:787158-787475 [Klebsiella variicola]
MFRIVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T179829 NZ_CP084895:c4543584-4543327 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10801.48 Da        Isoelectric Point: 10.2212

>AT179829 WP_023322192.1 NZ_CP063954:787468-787755 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANVTRASLDTLTR
YALACGIKHPQILLY

Download         Length: 288 bp

>AT179829 NZ_CP084895:c4543315-4542770 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H0BIE0

References