Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 67275..67872 Replicon chromosome
Accession NZ_CP063953
Organism Klebsiella variicola strain S131-2

Toxin (Protein)


Gene name higB Uniprot ID A0A1F2LYJ8
Locus tag ISD12_RS00285 Protein ID WP_023322191.1
Coordinates 67555..67872 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6H0BIE0
Locus tag ISD12_RS00280 Protein ID WP_023322192.1
Coordinates 67275..67562 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISD12_RS00250 63185..63433 + 249 WP_008805539.1 DUF1158 domain-containing protein -
ISD12_RS00255 63450..63791 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
ISD12_RS00260 63822..64937 - 1116 WP_194394748.1 MBL fold metallo-hydrolase -
ISD12_RS00265 65117..65698 + 582 WP_008805537.1 TetR/AcrR family transcriptional regulator -
ISD12_RS00270 65698..66066 + 369 WP_008805536.1 MmcQ/YjbR family DNA-binding protein -
ISD12_RS00275 66186..66839 + 654 WP_012968755.1 oxygen-insensitive NAD(P)H nitroreductase -
ISD12_RS00280 67275..67562 - 288 WP_023322192.1 helix-turn-helix transcriptional regulator Antitoxin
ISD12_RS00285 67555..67872 - 318 WP_023322191.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISD12_RS00290 68057..69100 - 1044 WP_016160437.1 DUF2157 domain-containing protein -
ISD12_RS00295 69763..70629 - 867 WP_022065427.1 helix-turn-helix transcriptional regulator -
ISD12_RS00300 70738..72165 + 1428 WP_131010146.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-89)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T179810 WP_023322191.1 NZ_CP063953:c67872-67555 [Klebsiella variicola]
MFRIVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T179810 NZ_CP084895:c1271086-1270979 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10801.48 Da        Isoelectric Point: 10.2212

>AT179810 WP_023322192.1 NZ_CP063953:c67562-67275 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANVTRASLDTLTR
YALACGIKHPQILLY

Download         Length: 288 bp

>AT179810 NZ_CP084895:1271133-1271199 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H0BIE0

References