Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 162122..162849 Replicon plasmid pS166-1.1
Accession NZ_CP063946
Organism Klebsiella pneumoniae strain S166-1

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag IOD25_RS26635 Protein ID WP_011251285.1
Coordinates 162538..162849 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IOD25_RS26630 Protein ID WP_011251286.1
Coordinates 162122..162541 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD25_RS26605 157439..157963 - 525 WP_071444207.1 hypothetical protein -
IOD25_RS26610 158156..158911 - 756 Protein_167 hypothetical protein -
IOD25_RS26615 159505..159968 + 464 Protein_168 hypothetical protein -
IOD25_RS26620 159968..160756 + 789 WP_114263119.1 hypothetical protein -
IOD25_RS26625 161010..161975 - 966 WP_194433533.1 IS5 family transposase -
IOD25_RS26630 162122..162541 - 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
IOD25_RS26635 162538..162849 - 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
IOD25_RS26640 163054..163512 - 459 Protein_173 DDE-type integrase/transposase/recombinase -
IOD25_RS26645 163605..163943 + 339 WP_023288266.1 IS66 family insertion sequence hypothetical protein -
IOD25_RS26650 163940..164287 + 348 WP_004114612.1 IS66 family insertion sequence element accessory protein TnpB -
IOD25_RS26655 164336..165385 + 1050 Protein_176 IS66 family transposase -
IOD25_RS26660 165443..166409 - 967 Protein_177 IS5 family transposase -
IOD25_RS26665 166429..167423 - 995 Protein_178 IS3 family transposase -
IOD25_RS26670 167578..167676 + 99 WP_004118688.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / qnrB4 / blaDHA-1 / mph(A) / aph(3')-Ia / sul2 / floR / tet(A) / blaLAP-2 / qnrS1 / dfrA14 / tet(D) - 1..433968 433968
- inside IScluster/Tn - - 139946..167342 27396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T179808 WP_011251285.1 NZ_CP063946:c162849-162538 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T179808 NZ_CP084895:c1270016-1269909 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT179808 WP_011251286.1 NZ_CP063946:c162541-162122 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT179808 NZ_CP084895:1270064-1270130 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1

Antitoxin

Source ID Structure

References