Detailed information of TA system TA179806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3926..4653
• Replicon: plasmid pS166-1.1
• Accession: NZ_CP063946
• Organism: Klebsiella pneumoniae strain S166-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: IOD25_RS25800
• Protein ID: WP_011251285.1
• Coordinates: 4342..4653 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IOD25_RS25795
• Protein ID: WP_011251286.1
• Coordinates: 3926..4345 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD25_RS25775	2..727	-	726	Protein_0	hypothetical protein	-
IOD25_RS25780	1320..1779	+	460	Protein_1	hypothetical protein	-
IOD25_RS25785	1779..2560	+	782	Protein_2	hypothetical protein	-
IOD25_RS25790	2814..3782	-	969	WP_194433524.1	IS5 family transposase	-
IOD25_RS25795	3926..4345	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
IOD25_RS25800	4342..4653	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
IOD25_RS25805	4858..5315	-	458	Protein_6	DDE-type integrase/transposase/recombinase	-
IOD25_RS25810	5408..5746	+	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
IOD25_RS25815	5743..6090	+	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
IOD25_RS25820	6138..7183	+	1046	Protein_9	IS66 family transposase	-
IOD25_RS25825	7239..8207	-	969	WP_194433525.1	IS5 family transposase	-
IOD25_RS25830	8227..9230	-	1004	Protein_11	IS3 family transposase	-
IOD25_RS25835	9378..9476	+	99	WP_004118688.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / qnrB4 / blaDHA-1 / mph(A) / aph(3')-Ia / sul2 / floR / tet(A) / blaLAP-2 / qnrS1 / dfrA14 / tet(D)	-	1..433968	433968
	-	inside	IScluster/Tn	-	-	2814..9257	6443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T179806 WP_011251285.1 NZ_CP063946:c4653-4342 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T179806 NZ_CP084895:c262893-262552 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT179806 WP_011251286.1 NZ_CP063946:c4345-3926 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT179806 NZ_CP084895:c263231-262914 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1