Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4641599..4642245 Replicon chromosome
Accession NZ_CP063945
Organism Klebsiella pneumoniae strain S166-1

Toxin (Protein)


Gene name higB Uniprot ID A0A231WWF9
Locus tag IOD25_RS22580 Protein ID WP_004174016.1
Coordinates 4641599..4641946 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag IOD25_RS22585 Protein ID WP_002920557.1
Coordinates 4641946..4642245 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD25_RS22570 4637525..4638958 + 1434 WP_002920564.1 glycogen synthase GlgA -
IOD25_RS22575 4638976..4641423 + 2448 WP_002920561.1 glycogen phosphorylase -
IOD25_RS22580 4641599..4641946 + 348 WP_004174016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IOD25_RS22585 4641946..4642245 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
IOD25_RS22590 4642308..4643816 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
IOD25_RS22595 4644021..4644350 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
IOD25_RS22600 4644401..4645231 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
IOD25_RS22605 4645281..4646039 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13518.55 Da        Isoelectric Point: 6.2327

>T179803 WP_004174016.1 NZ_CP063945:4641599-4641946 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T179803 NZ_CP084894:c4590148-4589807 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT179803 WP_002920557.1 NZ_CP063945:4641946-4642245 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT179803 NZ_CP084894:4590143-4590219 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231WWF9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References