Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 5115086..5115672 Replicon chromosome
Accession NZ_CP063943
Organism Klebsiella pneumoniae strain BS329-2

Toxin (Protein)


Gene name doc Uniprot ID -
Locus tag ISA55_RS24720 Protein ID WP_032421870.1
Coordinates 5115304..5115672 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag ISA55_RS24715 Protein ID WP_004174006.1
Coordinates 5115086..5115307 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA55_RS24695 5111243..5112169 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
ISA55_RS24700 5112166..5113443 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
ISA55_RS24705 5113440..5114207 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
ISA55_RS24710 5114209..5114922 + 714 WP_009484078.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
ISA55_RS24715 5115086..5115307 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ISA55_RS24720 5115304..5115672 + 369 WP_032421870.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ISA55_RS24725 5115945..5117261 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
ISA55_RS24730 5117368..5118255 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
ISA55_RS24735 5118252..5119097 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
ISA55_RS24740 5119099..5120169 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 5112166..5120906 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13570.96 Da        Isoelectric Point: 8.6410

>T179783 WP_032421870.1 NZ_CP063943:5115304-5115672 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAMSRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T179783 NZ_CP084894:c1271086-1270979 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT179783 WP_004174006.1 NZ_CP063943:5115086-5115307 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT179783 NZ_CP084894:1271133-1271199 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References