Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2401533..2402130 Replicon chromosome
Accession NZ_CP063939
Organism Klebsiella pneumoniae strain BM343

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag ISA62_RS11620 Protein ID WP_004142563.1
Coordinates 2401813..2402130 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag ISA62_RS11615 Protein ID WP_004142561.1
Coordinates 2401533..2401820 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA62_RS11585 2397613..2397861 + 249 WP_002893037.1 DUF1158 domain-containing protein -
ISA62_RS11590 2397879..2398220 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
ISA62_RS11595 2398251..2399366 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
ISA62_RS11600 2399546..2400127 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
ISA62_RS11605 2400127..2400495 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
ISA62_RS11610 2400615..2401268 + 654 WP_019704893.1 oxygen-insensitive NAD(P)H nitroreductase -
ISA62_RS11615 2401533..2401820 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
ISA62_RS11620 2401813..2402130 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISA62_RS11625 2402315..2403358 - 1044 WP_019704892.1 DUF2157 domain-containing protein -
ISA62_RS11630 2404028..2404894 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
ISA62_RS11635 2405003..2406430 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T179744 WP_004142563.1 NZ_CP063939:c2402130-2401813 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T179744 NZ_CP084892:c2153367-2153005 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT179744 WP_004142561.1 NZ_CP063939:c2401820-2401533 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT179744 NZ_CP084892:c2153534-2153364 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCTATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References