Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3664020..3664617 Replicon chromosome
Accession NZ_CP063936
Organism Klebsiella pneumoniae strain BS317-1

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag ISA63_RS17775 Protein ID WP_004142563.1
Coordinates 3664020..3664337 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag ISA63_RS17780 Protein ID WP_004142561.1
Coordinates 3664330..3664617 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA63_RS17760 3659723..3661150 - 1428 WP_004176980.1 MFS transporter -
ISA63_RS17765 3661259..3662125 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
ISA63_RS17770 3662792..3663835 + 1044 WP_087787624.1 DUF2157 domain-containing protein -
ISA63_RS17775 3664020..3664337 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISA63_RS17780 3664330..3664617 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
ISA63_RS17785 3664882..3665535 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
ISA63_RS17790 3665655..3666023 - 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
ISA63_RS17795 3666020..3666604 - 585 WP_002893026.1 TetR/AcrR family transcriptional regulator -
ISA63_RS17800 3666783..3667898 + 1116 WP_169546850.1 MBL fold metallo-hydrolase -
ISA63_RS17805 3667929..3668270 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
ISA63_RS17810 3668288..3668536 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T179732 WP_004142563.1 NZ_CP063936:3664020-3664337 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T179732 NZ_CP084879:c2555666-2555563 [Shigella flexneri]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT179732 WP_004142561.1 NZ_CP063936:3664330-3664617 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT179732 NZ_CP084879:2555562-2555796 [Shigella flexneri]
TAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCA
TTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAAT
AAAAAGCGCGGTGCTCATCAGCTTAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References