Detailed information of TA system TA179700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1226044..1226630
• Replicon: chromosome
• Accession: NZ_CP063934
• Organism: Klebsiella pneumoniae strain BS326-3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: ISA64_RS05775
• Protein ID: WP_032421870.1
• Coordinates: 1226262..1226630 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: ISA64_RS05770
• Protein ID: WP_004174006.1
• Coordinates: 1226044..1226265 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA64_RS05750	1222201..1223127	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
ISA64_RS05755	1223124..1224401	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
ISA64_RS05760	1224398..1225165	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
ISA64_RS05765	1225167..1225880	+	714	WP_009484078.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
ISA64_RS05770	1226044..1226265	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ISA64_RS05775	1226262..1226630	+	369	WP_032421870.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ISA64_RS05780	1226903..1228219	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
ISA64_RS05785	1228326..1229213	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
ISA64_RS05790	1229210..1230055	+	846	WP_004185988.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
ISA64_RS05795	1230057..1231127	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_009648 Klebsiella pneumoniae subsp. pneumoniae MGH 78578 Prophage TA022291 4164800..4173540 0.005	inside	Prophage	-	-	1223124..1231864	8740

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13570.96 Da
• Isoelectric Point: 8.6410

>T179700 WP_032421870.1 NZ_CP063934:1226262-1226630 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAMSRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T179700 NZ_CP084874:744176-744661 [Klebsiella africana]
ATGATTTCTGCCCCTGAGCCGCTTCACGCCGGGCATATTCTTACTCCATTCTGCAGTGGCGTGGATTCGATGGATAACTG
GCTGAAACAGCGGGCGATGAAGAACCAGCTTTCTGGCGCATCCCGCACGTTTGTCTGCTGCGACGATGCGAAGGTAATGG
CGTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCTGGTCGTTTTCGCCGCAATATGCCTGATCCGATC
CCGGTTGTGGTGCTGGGTCGTCTGGCGGTGGAGAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGCTGATGGTGACGTTGGGGGATTTGGTTCAT
GCCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT179700 WP_004174006.1 NZ_CP063934:1226044-1226265 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT179700 NZ_CP084874:743886-744179 [Klebsiella africana]
ATGCCAGCCGCAAACAGTATGACGATGAAACGCGAAACCTTGAACCTGCGCATCAAGCCCGCCGAGCGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGTTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGAGCCCAAT
GCCGCATTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAACAGGAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7