Detailed information of TA system TA179694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4659120..4659717
• Replicon: chromosome
• Accession: NZ_CP063933
• Organism: Klebsiella variicola strain BS369-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: ISA65_RS22450
• Protein ID: WP_012542526.1
• Coordinates: 4659120..4659437 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISA65_RS22455
• Protein ID: WP_012542525.1
• Coordinates: 4659430..4659717 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA65_RS22435	4654960..4656387	-	1428	WP_194387267.1	MFS transporter	-
ISA65_RS22440	4656496..4657362	+	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
ISA65_RS22445	4657892..4658935	+	1044	WP_023297181.1	hypothetical protein	-
ISA65_RS22450	4659120..4659437	+	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISA65_RS22455	4659430..4659717	+	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
ISA65_RS22460	4660203..4660856	-	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
ISA65_RS22465	4660976..4661344	-	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
ISA65_RS22470	4661344..4661925	-	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
ISA65_RS22475	4662105..4663220	+	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
ISA65_RS22480	4663251..4663592	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
ISA65_RS22485	4663609..4663857	-	249	WP_008805539.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T179694 WP_012542526.1 NZ_CP063933:4659120-4659437 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T179694 NZ_CP084873:3859962-3860180 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACACTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT179694 WP_012542525.1 NZ_CP063933:4659430-4659717 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT179694 NZ_CP084873:3859561-3859935 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2