Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 5070867..5071513 Replicon chromosome
Accession NZ_CP063932
Organism Klebsiella variicola strain BS375-3

Toxin (Protein)


Gene name higB Uniprot ID A0A1F2M0N0
Locus tag ISA66_RS24555 Protein ID WP_008806991.1
Coordinates 5071166..5071513 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag ISA66_RS24550 Protein ID WP_008806992.1
Coordinates 5070867..5071166 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA66_RS24530 5067074..5067832 - 759 WP_008806995.1 DeoR/GlpR family transcriptional regulator -
ISA66_RS24535 5067882..5068712 - 831 WP_008806994.1 rhomboid family intramembrane serine protease GlpG -
ISA66_RS24540 5068763..5069092 - 330 WP_023291211.1 thiosulfate sulfurtransferase GlpE -
ISA66_RS24545 5069296..5070804 + 1509 WP_008806993.1 glycerol-3-phosphate dehydrogenase -
ISA66_RS24550 5070867..5071166 - 300 WP_008806992.1 helix-turn-helix transcriptional regulator Antitoxin
ISA66_RS24555 5071166..5071513 - 348 WP_008806991.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISA66_RS24560 5071728..5074175 - 2448 WP_008806990.1 glycogen phosphorylase -
ISA66_RS24565 5074193..5075626 - 1434 WP_004202133.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13504.52 Da        Isoelectric Point: 6.2327

>T179675 WP_008806991.1 NZ_CP063932:c5071513-5071166 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T179675 NZ_CP084870:c3686939-3686837 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT179675 WP_008806992.1 NZ_CP063932:c5071166-5070867 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT179675 NZ_CP084870:3686830-3686975 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M0N0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References