Detailed information of TA system TA179663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1302615..1303212
• Replicon: chromosome
• Accession: NZ_CP063932
• Organism: Klebsiella variicola strain BS375-3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: ISA66_RS06085
• Protein ID: WP_012542526.1
• Coordinates: 1302615..1302932 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISA66_RS06090
• Protein ID: WP_012542525.1
• Coordinates: 1302925..1303212 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA66_RS06070	1298455..1299882	-	1428	WP_194387267.1	MFS transporter	-
ISA66_RS06075	1299991..1300857	+	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
ISA66_RS06080	1301387..1302430	+	1044	WP_023297181.1	hypothetical protein	-
ISA66_RS06085	1302615..1302932	+	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISA66_RS06090	1302925..1303212	+	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
ISA66_RS06095	1303698..1304351	-	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
ISA66_RS06100	1304471..1304839	-	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
ISA66_RS06105	1304839..1305420	-	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
ISA66_RS06110	1305600..1306715	+	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
ISA66_RS06115	1306746..1307087	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
ISA66_RS06120	1307104..1307352	-	249	WP_008805539.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T179663 WP_012542526.1 NZ_CP063932:1302615-1302932 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T179663 NZ_CP084868:c5297594-5297226 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT179663 WP_012542525.1 NZ_CP063932:1302925-1303212 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT179663 NZ_CP084868:c5297812-5297591 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2