Detailed information of TA system TA179631

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 138337..138763
• Replicon: plasmid pS183-1.2
• Accession: NZ_CP063929
• Organism: Klebsiella pneumoniae strain S183-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ISA68_RS26980
• Protein ID: WP_001312861.1
• Coordinates: 138337..138495 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	138539..138763 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA68_RS26945	133392..133619	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
ISA68_RS26950	133713..134399	-	687	WP_015059009.1	PAS domain-containing protein	-
ISA68_RS26955	134590..134973	-	384	WP_000124981.1	relaxosome protein TraM	-
ISA68_RS26960	135250..135897	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
ISA68_RS26965	136194..137015	-	822	WP_001234445.1	DUF945 domain-containing protein	-
ISA68_RS26970	137126..137422	-	297	WP_001272251.1	hypothetical protein	-
ISA68_RS26975	137722..138099	+	378	Protein_182	hypothetical protein	-
ISA68_RS26980	138337..138495	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	138539..138763	-	225	NuclAT_0	-	Antitoxin
-	138539..138763	-	225	NuclAT_0	-	Antitoxin
-	138539..138763	-	225	NuclAT_0	-	Antitoxin
-	138539..138763	-	225	NuclAT_0	-	Antitoxin
ISA68_RS26985	138775..139494	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
ISA68_RS26990	139491..139925	-	435	WP_194443810.1	conjugation system SOS inhibitor PsiB	-
ISA68_RS26995	139994..142017	-	2024	Protein_186	ParB/RepB/Spo0J family partition protein	-
ISA68_RS27000	142078..142311	-	234	WP_000006003.1	DUF905 family protein	-
ISA68_RS27005	142369..142896	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / aac(3)-IId / erm(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-55 / blaTEM-1B / fosA3 / rmtB / sitABCD / ARR-3 / catB3	iutA / iucD / iucC / iucB / iucA	1..190929	190929
	-	inside	Integron	-	iutA / iucD / iucC / iucB / iucA	364..190928	190564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T179631 WP_001312861.1 NZ_CP063929:c138495-138337 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T179631 NZ_CP084860:c2571008-2569686 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT179631 NZ_CP063929:c138763-138539 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin