Detailed information of TA system TA179627

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 98866..99119
• Replicon: plasmid pS183-1.2
• Accession: NZ_CP063929
• Organism: Klebsiella pneumoniae strain S183-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: ISA68_RS26750
• Protein ID: WP_001312851.1
• Coordinates: 98866..99015 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	99060..99119 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA68_RS26715	94230..94645	-	416	Protein_130	IS1 family transposase	-
ISA68_RS26720	94894..95295	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
ISA68_RS26725	95228..95485	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
ISA68_RS26730	95575..95907	-	333	WP_072134990.1	CPBP family intramembrane metalloprotease	-
ISA68_RS26735	97165..98022	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
ISA68_RS26740	98015..98089	-	75	WP_001375168.1	RepA leader peptide Tap	-
ISA68_RS26745	98334..98582	-	249	WP_000083837.1	replication regulatory protein RepA	-
ISA68_RS26750	98866..99015	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	99060..99119	+	60	NuclAT_4	-	Antitoxin
-	99060..99119	+	60	NuclAT_4	-	Antitoxin
-	99060..99119	+	60	NuclAT_4	-	Antitoxin
-	99060..99119	+	60	NuclAT_4	-	Antitoxin
ISA68_RS26755	99320..99550	-	231	WP_001736714.1	hypothetical protein	-
ISA68_RS26760	99714..100313	-	600	WP_032083981.1	hypothetical protein	-
ISA68_RS26765	100699..100899	-	201	WP_015059022.1	hypothetical protein	-
ISA68_RS26770	101031..101591	-	561	WP_000139328.1	fertility inhibition protein FinO	-
ISA68_RS26775	101646..102392	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / aac(3)-IId / erm(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-55 / blaTEM-1B / fosA3 / rmtB / sitABCD / ARR-3 / catB3	iutA / iucD / iucC / iucB / iucA	1..190929	190929
	-	inside	Integron	-	iutA / iucD / iucC / iucB / iucA	364..190928	190564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T179627 WP_001312851.1 NZ_CP063929:c99015-98866 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T179627 NZ_CP084860:331780-332148 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGTATTACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT179627 NZ_CP063929:99060-99119 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin