Detailed information of TA system TA179623

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66594..66827
• Replicon: plasmid pS183-1.2
• Accession: NZ_CP063929
• Organism: Klebsiella pneumoniae strain S183-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ISA68_RS26515
• Protein ID: WP_001312861.1
• Coordinates: 66594..66752 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	66796..66827 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA68_RS26490	61968..62657	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
ISA68_RS26495	62844..63227	-	384	WP_001151524.1	relaxosome protein TraM	-
ISA68_RS26500	63548..64150	+	603	WP_194443813.1	transglycosylase SLT domain-containing protein	-
ISA68_RS26505	64447..65268	-	822	WP_001234426.1	DUF945 domain-containing protein	-
ISA68_RS26510	65386..65673	-	288	WP_000107535.1	hypothetical protein	-
ISA68_RS26515	66594..66752	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	66796..66827	-	32	NuclAT_2	-	Antitoxin
-	66796..66827	-	32	NuclAT_2	-	Antitoxin
-	66796..66827	-	32	NuclAT_2	-	Antitoxin
-	66796..66827	-	32	NuclAT_2	-	Antitoxin
-	68269..68466	-	198	NuclAT_1	-	-
-	68269..68466	-	198	NuclAT_1	-	-
-	68269..68466	-	198	NuclAT_1	-	-
-	68269..68466	-	198	NuclAT_1	-	-
ISA68_RS26525	68478..69197	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
ISA68_RS26530	69194..69628	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
ISA68_RS26535	69683..69880	-	198	Protein_94	hypothetical protein	-
ISA68_RS26540	69908..70141	-	234	WP_000005990.1	DUF905 family protein	-
ISA68_RS26545	70209..70748	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
ISA68_RS26550	70774..70980	-	207	WP_194443814.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / aac(3)-IId / erm(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-55 / blaTEM-1B / fosA3 / rmtB / sitABCD / ARR-3 / catB3	iutA / iucD / iucC / iucB / iucA	1..190929	190929
	-	inside	Integron	-	iutA / iucD / iucC / iucB / iucA	364..190928	190564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T179623 WP_001312861.1 NZ_CP063929:c66752-66594 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T179623 NZ_CP084859:c4735051-4734719 [Klebsiella pneumoniae]
ATGAAAACTTTACCTGCAACAACTCCGCAGACGGCGAAGCTATGTCTGACGTCCGCGGATACCTGGCAAATGTTACTGAC
ACGTCTGCTAGAACAGCATTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCAGGGATCCCTCTGGCCGATGCAGTTAACTTTCTGGTGGATAAGTACGCACTGGTTCGTATCGATCGCAGAGGATTG
AGTTGGCAGGAGCAATCATCCTATCTCCGGCTTGTCGATACTCAGCGAACCATTAAGGTTATTGAGTTGTGGCTATTTGG
TGTAATGATGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT179623 NZ_CP063929:c66827-66796 [Klebsiella pneumoniae]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin