Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 3598612..3599198 Replicon chromosome
Accession NZ_CP063927
Organism Klebsiella pneumoniae strain S183-1

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag ISA68_RS17265 Protein ID WP_002920800.1
Coordinates 3598830..3599198 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag ISA68_RS17260 Protein ID WP_004174006.1
Coordinates 3598612..3598833 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA68_RS17240 3594770..3595696 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
ISA68_RS17245 3595693..3596970 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
ISA68_RS17250 3596967..3597734 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
ISA68_RS17255 3597736..3598449 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
ISA68_RS17260 3598612..3598833 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ISA68_RS17265 3598830..3599198 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ISA68_RS17270 3599471..3600787 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
ISA68_RS17275 3600894..3601781 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
ISA68_RS17280 3601778..3602623 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
ISA68_RS17285 3602625..3603695 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3593599..3604432 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T179605 WP_002920800.1 NZ_CP063927:3598830-3599198 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T179605 NZ_CP084856:1994967-1995069 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT179605 WP_004174006.1 NZ_CP063927:3598612-3598833 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT179605 NZ_CP084856:c1995076-1994931 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References