Detailed information of TA system TA179591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2349516..2350113
• Replicon: chromosome
• Accession: NZ_CP063926
• Organism: Klebsiella variicola strain S187-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: ISA69_RS11335
• Protein ID: WP_012542526.1
• Coordinates: 2349796..2350113 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISA69_RS11330
• Protein ID: WP_012542525.1
• Coordinates: 2349516..2349803 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA69_RS11300	2345426..2345674	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
ISA69_RS11305	2345691..2346032	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
ISA69_RS11310	2346063..2347178	-	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
ISA69_RS11315	2347358..2347939	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
ISA69_RS11320	2347939..2348307	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
ISA69_RS11325	2348427..2349080	+	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
ISA69_RS11330	2349516..2349803	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
ISA69_RS11335	2349796..2350113	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISA69_RS11340	2350298..2351341	-	1044	WP_016160437.1	DUF2157 domain-containing protein	-
ISA69_RS11345	2351871..2352737	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
ISA69_RS11350	2352846..2354273	+	1428	WP_023297179.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T179591 WP_012542526.1 NZ_CP063926:c2350113-2349796 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T179591 NZ_CP084852:323751-324119 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT179591 WP_012542525.1 NZ_CP063926:c2349803-2349516 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT179591 NZ_CP084852:323533-323754 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2