Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 9231..9902 Replicon plasmid pBM337-1
Accession NZ_CP063920
Organism Klebsiella pneumoniae strain BM337-1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag ISA74_RS25695 Protein ID WP_012539985.1
Coordinates 9579..9902 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ISA74_RS25690 Protein ID WP_194432919.1
Coordinates 9231..9530 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA74_RS25660 5308..6474 + 1167 WP_194432914.1 IS21 family transposase -
ISA74_RS25665 6474..7279 + 806 Protein_6 ATP-binding protein -
ISA74_RS25670 7326..7502 + 177 Protein_7 transposase -
ISA74_RS25675 7731..8126 + 396 WP_194432916.1 TonB family protein -
ISA74_RS25680 8087..8623 + 537 Protein_9 GNAT family N-acetyltransferase -
ISA74_RS25685 8911..9192 + 282 WP_194432918.1 hypothetical protein -
ISA74_RS25690 9231..9530 - 300 WP_194432919.1 helix-turn-helix domain-containing protein Antitoxin
ISA74_RS25695 9579..9902 - 324 WP_012539985.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISA74_RS25700 10032..10675 + 644 Protein_13 IS110 family transposase -
ISA74_RS25705 10972..11243 + 272 Protein_14 transposase -
ISA74_RS25710 11277..12374 - 1098 Protein_15 IS3 family transposase -
ISA74_RS25715 13190..13366 + 177 Protein_16 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..36933 36933
- inside IScluster/Tn - - 5308..12028 6720


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(45-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-82)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12358.27 Da        Isoelectric Point: 9.6240

>T179522 WP_012539985.1 NZ_CP063920:c9902-9579 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDDLSEKEKAVMKALTQQLK

Download         Length: 324 bp

>T179522 NZ_CP084819:28275-28580 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11155.05 Da        Isoelectric Point: 6.8718

>AT179522 WP_194432919.1 NZ_CP063920:c9530-9231 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRIPSGIALKM
LCLIEREPALIDTLRGIHV

Download         Length: 300 bp

>AT179522 NZ_CP084819:28055-28273 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References