Detailed information of TA system TA179503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 975341..975987
• Replicon: chromosome
• Accession: NZ_CP063919
• Organism: Klebsiella pneumoniae strain BM337-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ISA74_RS04835
• Protein ID: WP_194429468.1
• Coordinates: 975640..975987 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISA74_RS04830
• Protein ID: WP_194429469.1
• Coordinates: 975341..975640 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA74_RS04810	971547..972305	-	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-
ISA74_RS04815	972355..973185	-	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
ISA74_RS04820	973236..973565	-	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
ISA74_RS04825	973770..975278	+	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
ISA74_RS04830	975341..975640	-	300	WP_194429469.1	helix-turn-helix transcriptional regulator	Antitoxin
ISA74_RS04835	975640..975987	-	348	WP_194429468.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISA74_RS04840	976163..978610	-	2448	WP_002920561.1	glycogen phosphorylase	-
ISA74_RS04845	978628..980061	-	1434	WP_002920564.1	glycogen synthase GlgA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (17-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13419.41 Da
• Isoelectric Point: 6.2327

>T179503 WP_194429468.1 NZ_CP063919:c975987-975640 [Klebsiella pneumoniae]
MWDVETTDTFDTGFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T179503 NZ_CP084804:c311749-311465 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10813.63 Da
• Isoelectric Point: 10.6143

>AT179503 WP_194429469.1 NZ_CP063919:c975640-975341 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAERRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNAPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT179503 NZ_CP084804:c311987-311739 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA