Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 202769..203443 Replicon plasmid pBM374-1
Accession NZ_CP063918
Organism Klebsiella variicola strain BM374-1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag ISA75_RS27690 Protein ID WP_012539985.1
Coordinates 202769..203092 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ISA75_RS27695 Protein ID WP_012540018.1
Coordinates 203141..203443 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA75_RS27670 199291..199467 - 177 Protein_174 IS3 family transposase -
ISA75_RS27675 200290..201389 + 1100 WP_172870690.1 IS3 family transposase -
ISA75_RS27680 201423..201695 - 273 Protein_176 transposase -
ISA75_RS27685 201993..202670 - 678 Protein_177 IS110 family transposase -
ISA75_RS27690 202769..203092 + 324 WP_012539985.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISA75_RS27695 203141..203443 + 303 WP_012540018.1 helix-turn-helix domain-containing protein Antitoxin
ISA75_RS27700 203479..203769 - 291 WP_012540036.1 hypothetical protein -
ISA75_RS27705 204058..204600 - 543 WP_012539969.1 GNAT family N-acetyltransferase -
ISA75_RS27710 204625..204960 - 336 WP_032700858.1 TonB family protein -
ISA75_RS27715 205186..205347 - 162 Protein_183 transposase -
ISA75_RS27720 205420..206220 - 801 WP_080929196.1 IS21-like element helper ATPase IstB -
ISA75_RS27725 206220..207390 - 1171 Protein_185 IS21 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..303115 303115
- inside IScluster/Tn - - 200290..207366 7076


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(45-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-83)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12358.27 Da        Isoelectric Point: 9.6240

>T179501 WP_012539985.1 NZ_CP063918:202769-203092 [Klebsiella variicola]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDDLSEKEKAVMKALTQQLK

Download         Length: 324 bp

>T179501 NZ_CP084804:c95578-95291 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11226.13 Da        Isoelectric Point: 6.8718

>AT179501 WP_012540018.1 NZ_CP063918:203141-203443 [Klebsiella variicola]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRIPSGIALK
MLCLIEREPALIDTLRGIHV

Download         Length: 303 bp

>AT179501 NZ_CP084804:c95817-95578 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References