Detailed information of TA system TA179473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2319954..2320551
• Replicon: chromosome
• Accession: NZ_CP063916
• Organism: Klebsiella variicola strain BS327-2-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: ISA76_RS11130
• Protein ID: WP_012542526.1
• Coordinates: 2319954..2320271 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISA76_RS11135
• Protein ID: WP_012542525.1
• Coordinates: 2320264..2320551 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA76_RS11115	2315794..2317221	-	1428	WP_194387267.1	MFS transporter	-
ISA76_RS11120	2317330..2318196	+	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
ISA76_RS11125	2318726..2319769	+	1044	WP_023297181.1	hypothetical protein	-
ISA76_RS11130	2319954..2320271	+	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISA76_RS11135	2320264..2320551	+	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
ISA76_RS11140	2321037..2321690	-	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
ISA76_RS11145	2321810..2322178	-	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
ISA76_RS11150	2322178..2322759	-	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
ISA76_RS11155	2322939..2324054	+	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
ISA76_RS11160	2324085..2324426	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
ISA76_RS11165	2324443..2324691	-	249	WP_008805539.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T179473 WP_012542526.1 NZ_CP063916:2319954-2320271 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T179473 NZ_CP084776:59035-59451 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCGTCCTGGTGACAAACAATGTGAGAGAGTTTGAGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT179473 WP_012542525.1 NZ_CP063916:2320264-2320551 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT179473 NZ_CP084776:58808-59038 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATTCGGCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2