Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1107110..1107756 Replicon chromosome
Accession NZ_CP063916
Organism Klebsiella variicola strain BS327-2-1

Toxin (Protein)


Gene name higB Uniprot ID A0A1F2M0N0
Locus tag ISA76_RS05365 Protein ID WP_008806991.1
Coordinates 1107110..1107457 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag ISA76_RS05370 Protein ID WP_008806992.1
Coordinates 1107457..1107756 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA76_RS05355 1102997..1104430 + 1434 WP_004202133.1 glycogen synthase GlgA -
ISA76_RS05360 1104448..1106895 + 2448 WP_008806990.1 glycogen phosphorylase -
ISA76_RS05365 1107110..1107457 + 348 WP_008806991.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISA76_RS05370 1107457..1107756 + 300 WP_008806992.1 helix-turn-helix transcriptional regulator Antitoxin
ISA76_RS05375 1107819..1109327 - 1509 WP_008806993.1 glycerol-3-phosphate dehydrogenase -
ISA76_RS05380 1109531..1109860 + 330 WP_023291211.1 thiosulfate sulfurtransferase GlpE -
ISA76_RS05385 1109911..1110741 + 831 WP_008806994.1 rhomboid family intramembrane serine protease GlpG -
ISA76_RS05390 1110791..1111549 + 759 WP_008806995.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13504.52 Da        Isoelectric Point: 6.2327

>T179470 WP_008806991.1 NZ_CP063916:1107110-1107457 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T179470 NZ_CP084774:105670-105981 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT179470 WP_008806992.1 NZ_CP063916:1107457-1107756 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT179470 NZ_CP084774:105978-106397 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M0N0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References