Detailed information of TA system TA179453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1443724..1444321
• Replicon: chromosome
• Accession: NZ_CP063915
• Organism: Klebsiella variicola strain M186-1-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: ISA77_RS06785
• Protein ID: WP_012542526.1
• Coordinates: 1443724..1444041 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISA77_RS06790
• Protein ID: WP_012542525.1
• Coordinates: 1444034..1444321 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA77_RS06770	1439564..1440991	-	1428	WP_023297179.1	MFS transporter	-
ISA77_RS06775	1441100..1441966	+	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
ISA77_RS06780	1442496..1443539	+	1044	WP_016160437.1	DUF2157 domain-containing protein	-
ISA77_RS06785	1443724..1444041	+	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISA77_RS06790	1444034..1444321	+	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
ISA77_RS06795	1444757..1445410	-	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
ISA77_RS06800	1445530..1445898	-	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
ISA77_RS06805	1445898..1446479	-	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
ISA77_RS06810	1446659..1447774	+	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
ISA77_RS06815	1447805..1448146	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
ISA77_RS06820	1448163..1448411	-	249	WP_008805539.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T179453 WP_012542526.1 NZ_CP063915:1443724-1444041 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T179453 NZ_CP084767:293172-293447 [Klebsiella variicola subsp. tropica]
ATGGACCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCACACGCTGGAGCAACTGTTTAAAATCCCTGTCCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTGGGCGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCACCCTTCGCCGGATACCGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT179453 WP_012542525.1 NZ_CP063915:1444034-1444321 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT179453 NZ_CP084767:293444-293803 [Klebsiella variicola subsp. tropica]
ATGATCAAACCTAAAACGCCAAACTCAATGGAGATTGCCGGCCAGCCTGCCGTTATCAATTACGTGCCTGAGCTCAATGC
ATTCCGTGGTAAGTTCCTGGGATTATCAGGATATTGCGATTTTGTTTCCGATAGCATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAATATCTCGCAGATTGTCATGAAGCGGGGATTGAGCCTTATGCTCACCCGGAAAAGCTGAAAACC
TTCACGCTGCGCTATCCTGAGTCATTTGGTGAGCGTCTTAGCTCCGCCGCTGCGGAGGAACAGGTTTCCGTAAATACCTG
GATCCTCGAGACGCTCAATGAACGTCTCAAACAGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2