Detailed information of TA system TA179418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3375772..3376418
• Replicon: chromosome
• Accession: NZ_CP063912
• Organism: Klebsiella variicola strain BS359-2-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1F2M0N0
• Locus tag: ISA79_RS16115
• Protein ID: WP_008806991.1
• Coordinates: 3375772..3376119 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1F2LZT5
• Locus tag: ISA79_RS16120
• Protein ID: WP_008806992.1
• Coordinates: 3376119..3376418 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA79_RS16105	3371659..3373092	+	1434	WP_004202133.1	glycogen synthase GlgA	-
ISA79_RS16110	3373110..3375557	+	2448	WP_008806990.1	glycogen phosphorylase	-
ISA79_RS16115	3375772..3376119	+	348	WP_008806991.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISA79_RS16120	3376119..3376418	+	300	WP_008806992.1	helix-turn-helix transcriptional regulator	Antitoxin
ISA79_RS16125	3376481..3377989	-	1509	WP_008806993.1	glycerol-3-phosphate dehydrogenase	-
ISA79_RS16130	3378193..3378522	+	330	WP_023291211.1	thiosulfate sulfurtransferase GlpE	-
ISA79_RS16135	3378573..3379403	+	831	WP_008806994.1	rhomboid family intramembrane serine protease GlpG	-
ISA79_RS16140	3379453..3380211	+	759	WP_008806995.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13504.52 Da
• Isoelectric Point: 6.2327

>T179418 WP_008806991.1 NZ_CP063912:3375772-3376119 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T179418 NZ_CP084742:421484-422056 [Pseudomonas sp. L5B5]
ATGAACAGCGAACTTCTCACCGGCCGCCTCGAGTTTCTCCGTGAAGCGGAACGGCTCAAGGATGTGTTGCGCAGTGCCCA
CACCTCCAGCGGTCGCCAGGAAAGCACCGCCGAACACAGTTGGCGCCTGTGCCTGATGGCGCTGTTGCTGGAGGACCAGC
TGGAGGATGTGGACCTGCTGCAGGTCCTGAAAATGTGCATCGTGCATGACCTGGGGGAAGCGCTCCATGGCGACATCCCG
GCGGTGGAGCAAGCGGCCCATCCGGACAAGGGCCAGCAGGAGCGCGAAGACTTGCAGACCCTCGCCAGCTGCCTCGACGC
TCCGGCCCGCCAGCGCCTGCTGGGCCTGTGGGACGAGTACGAGGCTGCCAGCTCGGCCGAGGCTCGTACCGTCAAGGCGC
TGGACAAGCTGGAGACCCTGTTGCAGCACAACCAGGGACGCAACCCCGCGGATTTCGACTATGGGTTCAACCTCGACTAC
GGTCGTCGCTACACCAGTGCCAGTCCATTGTTCGAGGCCCTGCGCGAACAGATCGACCGCGACACCCAGCGCCACCTCGC
TGCACGCAAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10814.61 Da
• Isoelectric Point: 10.1464

>AT179418 WP_008806992.1 NZ_CP063912:3376119-3376418 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT179418 NZ_CP084742:420824-421369 [Pseudomonas sp. L5B5]
ATGTTCACTTCCCCCCACCTGGGCCTGGCCCTGCGCCGCTGGCGCCTGCTGCACCGGGTCAAGCAACAGCACGCCGCCGA
ACTGTTCGGAGTGGCGCAGTCGAGCATTTCCCGCTGGGAAAACGGCCAGCAGGCCATGGAACCGGCCGAGCGGGCGAAGC
TGGAGCAGTTGCTGGCGGCCAACCTCAGCGCTGCCGCCGACCAGGCCCTGGCCCGGCTGGTGAACGACAGCCCGCGCGCG
GTGCACCTGGTGTGCGACCTGACTCATCGCCTGCTGGCCAGTTCGCCGACCCGCGCCGGGCAGTTCGGCGTACCACTGAG
CGAGCTGCTGGGGCAGTCGCTGTGGGGCTTCTGCACCGAGGAAATCGTGCGCAAGGAAGCGGCTCTCGATGACCTGGGCT
GGCGCGAAGTGCTGGCGCCAGCGGCGCTGGAGTTCTTCAGCGGGCACAACGGGTCGGCCATCGTGCCCATCGCCCCAAGC
CAATGTCGCTGGACCCGCCTGAACCTGTCCGATGGCAGCGCGGTACGCCTGGTCGAAACTCTCTGA

Structures

Toxin

• AlphaFold DB: A0A1F2M0N0

Antitoxin

• AlphaFold DB: A0A1F2LZT5