Detailed information of TA system TA179413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1594275..1594872
• Replicon: chromosome
• Accession: NZ_CP063912
• Organism: Klebsiella variicola strain BS359-2-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: ISA79_RS07795
• Protein ID: WP_012542526.1
• Coordinates: 1594555..1594872 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISA79_RS07790
• Protein ID: WP_012542525.1
• Coordinates: 1594275..1594562 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA79_RS07760	1590135..1590383	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
ISA79_RS07765	1590400..1590741	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
ISA79_RS07770	1590772..1591887	-	1116	WP_162499993.1	MBL fold metallo-hydrolase	-
ISA79_RS07775	1592067..1592648	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
ISA79_RS07780	1592648..1593016	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
ISA79_RS07785	1593136..1593789	+	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
ISA79_RS07790	1594275..1594562	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
ISA79_RS07795	1594555..1594872	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISA79_RS07800	1595057..1596100	-	1044	WP_023297181.1	hypothetical protein	-
ISA79_RS07805	1596630..1597496	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
ISA79_RS07810	1597605..1599032	+	1428	WP_042948136.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T179413 WP_012542526.1 NZ_CP063912:c1594872-1594555 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T179413 NZ_CP084741:c1546645-1546226 [Pectobacterium carotovorum]
ATGACAGATATCTTGCTCAACAAAAGCAGCACCATACAAAGATGCCTACGTCGTATTCGTGAGGAATTCGATACTGAGGA
AGGGTTTCGTCAAAACTTCACCAAGCAAGATTCCGTCATACTTAACATACAGCGTGCCTGCGAAGCCGCTATTGATATCG
CTAATTATCTTATCCGTATCAAGCAGCTAGGCATACCACAAAGTAGCCGCGATAGCTTCGCGCTTCTTGCTGCAAACCAG
ATTATTACGATTGATCTGTCTGATAATTTGCAAAAAATGGTGGGATTAAGAAATATTGCCGTGCATGACTATCAGGCGCT
CAATCTGGATATTGTCATCCACGTCATTTCTCATCGTTTAAGCGATTTTGAACATTTTATCAAACAAATAGCCCGGTACA
GCGACAGCCAGCACCTTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT179413 WP_012542525.1 NZ_CP063912:c1594562-1594275 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT179413 NZ_CP084741:c1547025-1546642 [Pectobacterium carotovorum]
ATGTTCGATAAGATCGTTTTGACACTGAAAAAACAGATGCCCGACATCAAAATTATTTATCTCTTTGGTTCTCAGGCTAC
AGGCAATGCCAGAGCGGACAGCGACATTGATATCGCCATCATGGCTACAAGAGCACTGGATCCCGTTGAACGCTGGGAGC
TATCTAATCAATTAGCAAAGGAAGTGGGTCATGACGTCGATCTTATCGATTTATTGCAGGCTTCAACGGTATTAAAAATG
GAGATCGTTCGTAACGGCAAGCTGCTTTATGATGCGGAAACAGCAGCGGGAGAATTTGAAATGACAACACTTTCAATGTA
CCAGCATCTGCAAAAAGAGCGTGCGGATATCATTCGTAGCTTTAATCAGGATCTAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2