Detailed information of TA system TA179411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4207972..4208569
• Replicon: chromosome
• Accession: NZ_CP063911
• Organism: Klebsiella variicola strain M186-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: ISA80_RS20320
• Protein ID: WP_012542526.1
• Coordinates: 4207972..4208289 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISA80_RS20325
• Protein ID: WP_012542525.1
• Coordinates: 4208282..4208569 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA80_RS20305	4203812..4205239	-	1428	WP_023297179.1	MFS transporter	-
ISA80_RS20310	4205348..4206214	+	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
ISA80_RS20315	4206744..4207787	+	1044	WP_016160437.1	DUF2157 domain-containing protein	-
ISA80_RS20320	4207972..4208289	+	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISA80_RS20325	4208282..4208569	+	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
ISA80_RS20330	4209005..4209658	-	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
ISA80_RS20335	4209778..4210146	-	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
ISA80_RS20340	4210146..4210727	-	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
ISA80_RS20345	4210907..4212022	+	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
ISA80_RS20350	4212053..4212394	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
ISA80_RS20355	4212411..4212659	-	249	WP_008805539.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T179411 WP_012542526.1 NZ_CP063911:4207972-4208289 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T179411 NZ_CP084741:c1039319-1039014 [Pectobacterium carotovorum]
ATGCAATTCATTGTTTATCAATACAAACGCGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCAAAGCGGCGTATGGTCATCCCGCTTATCGAAGCACATCACCTGTCTGAGAAAGTGAATAAAACATTGTTCCCTTTGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACCACTGAGCTATCGAGCGTTCCCGTTGAGGTCATAGGTGAAGAAATT
GCGGATCTTGGCGAGTATGCGGATGAGATTAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT179411 WP_012542525.1 NZ_CP063911:4208282-4208569 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT179411 NZ_CP084741:c1039540-1039322 [Pectobacterium carotovorum]
ATGAAGCACCGCGTTAGCGTTACCGTGGACAAAGACAATTATCAGATTCTGAGTGCTGCCGGAGTCAATATTTCTGGGCT
GGTGAATGATGTCATTGGCAAAGAAGCTCGCCGCATCAAGGCTGAAGAGTGGAAGAAGGAAAACCGCGAAGGAATGGAAG
AAGTCGCCCGATTTATCGCGCAGCATGGCTCCTTTGCGGATGAAAACAGGAGCTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2