Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3022820..3023466 Replicon chromosome
Accession NZ_CP063910
Organism Klebsiella variicola strain BS325-2

Toxin (Protein)


Gene name higB Uniprot ID A0A1F2M0N0
Locus tag ISA81_RS14935 Protein ID WP_008806991.1
Coordinates 3023119..3023466 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag ISA81_RS14930 Protein ID WP_008806992.1
Coordinates 3022820..3023119 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA81_RS14910 3019027..3019785 - 759 WP_008806995.1 DeoR/GlpR family transcriptional regulator -
ISA81_RS14915 3019835..3020665 - 831 WP_008806994.1 rhomboid family intramembrane serine protease GlpG -
ISA81_RS14920 3020716..3021045 - 330 WP_023291211.1 thiosulfate sulfurtransferase GlpE -
ISA81_RS14925 3021249..3022757 + 1509 WP_008806993.1 glycerol-3-phosphate dehydrogenase -
ISA81_RS14930 3022820..3023119 - 300 WP_008806992.1 helix-turn-helix transcriptional regulator Antitoxin
ISA81_RS14935 3023119..3023466 - 348 WP_008806991.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISA81_RS14940 3023681..3026128 - 2448 WP_008806990.1 glycogen phosphorylase -
ISA81_RS14945 3026146..3027579 - 1434 WP_004202133.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13504.52 Da        Isoelectric Point: 6.2327

>T179389 WP_008806991.1 NZ_CP063910:c3023466-3023119 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T179389 NZ_CP084735:c25351-24992 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGTCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTATGGAGATAAATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT179389 WP_008806992.1 NZ_CP063910:c3023119-3022820 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT179389 NZ_CP084735:c24992-24693 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M0N0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References