Detailed information of TA system TA179375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 30643..31370
• Replicon: plasmid pM212-2
• Accession: NZ_CP063909
• Organism: Klebsiella pneumoniae strain M212-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: ISA82_RS26150
• Protein ID: WP_011251285.1
• Coordinates: 31059..31370 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISA82_RS26145
• Protein ID: WP_011251286.1
• Coordinates: 30643..31062 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA82_RS26115	25811..26281	-	471	WP_048333570.1	hypothetical protein	-
ISA82_RS26120	26474..27229	-	756	Protein_24	hypothetical protein	-
ISA82_RS26125	27825..28268	+	444	WP_048333569.1	hypothetical protein	-
ISA82_RS26130	28289..29077	+	789	WP_040217257.1	hypothetical protein	-
ISA82_RS26135	29091..29426	+	336	Protein_27	conjugal transfer protein TraI	-
ISA82_RS26140	29528..30496	-	969	WP_074428168.1	IS5 family transposase	-
ISA82_RS26145	30643..31062	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
ISA82_RS26150	31059..31370	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
ISA82_RS26155	31575..32033	-	459	Protein_31	DDE-type integrase/transposase/recombinase	-
ISA82_RS26160	32147..32844	+	698	Protein_32	IS1 family transposase	-
ISA82_RS26165	32861..33295	+	435	Protein_33	thioredoxin fold domain-containing protein	-
ISA82_RS26170	33312..33623	+	312	WP_011251282.1	hypothetical protein	-
ISA82_RS26175	33637..33978	+	342	WP_011251281.1	hypothetical protein	-
ISA82_RS26180	34038..34994	+	957	WP_011251280.1	DsbA family protein	-
ISA82_RS26185	35111..35686	+	576	WP_074424927.1	hypothetical protein	-
ISA82_RS26190	35794..36309	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..214844	214844
	-	inside	IScluster/Tn	-	-	24821..32844	8023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T179375 WP_011251285.1 NZ_CP063909:c31370-31059 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T179375 NZ_CP084721:448380-448769 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT179375 WP_011251286.1 NZ_CP063909:c31062-30643 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT179375 NZ_CP084721:448116-448373 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1