Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 3824682..3825268 Replicon chromosome
Accession NZ_CP063908
Organism Klebsiella pneumoniae strain M212-2

Toxin (Protein)


Gene name doc Uniprot ID A0A330WAT7
Locus tag ISA82_RS18515 Protein ID WP_014906830.1
Coordinates 3824682..3825050 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag ISA82_RS18520 Protein ID WP_004174006.1
Coordinates 3825047..3825268 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA82_RS18495 3820185..3821255 - 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
ISA82_RS18500 3821257..3822102 - 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
ISA82_RS18505 3822099..3822986 - 888 WP_014906831.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
ISA82_RS18510 3823093..3824409 - 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
ISA82_RS18515 3824682..3825050 - 369 WP_014906830.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ISA82_RS18520 3825047..3825268 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ISA82_RS18525 3825432..3826145 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
ISA82_RS18530 3826147..3826914 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
ISA82_RS18535 3826911..3828188 - 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
ISA82_RS18540 3828185..3829111 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3819448..3828188 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(28-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13537.95 Da        Isoelectric Point: 8.6410

>T179371 WP_014906830.1 NZ_CP063908:c3825050-3824682 [Klebsiella pneumoniae]
MTLQIISAEEIILFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T179371 NZ_CP084714:3421584-3421865 [Pseudomonas asiatica]
ATGACCTATAAACTCGAATTCCTGCCTTCGGCACTGAAAGAGTGGGGCAAGCTAGGCCACACCGTGCGCGAACAGATCAA
GAAAAAACTCGCCGAGCGCCTGCAGTCACCCAAGGTACAGGCCGACGCGCTGCGTGACCTGCCGAACCACTACAAAATCA
AGCTCAAGGCCTCTGGATATCGCCTCGTCTATCGAGTCGAGGACGAGCGGATCGTTGTAGTGGTGGTATCGGTTGGCAAA
CGCGAGCGTAGCGAGGTGTATCGATCCGCTCAGAAGCGGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT179371 WP_004174006.1 NZ_CP063908:c3825268-3825047 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT179371 NZ_CP084714:3421349-3421594 [Pseudomonas asiatica]
ATGCAAAACATATTCGCTGACGTGGCCGTAAGCGTGTCGGAACTGAAAAAGAACCCATCCGCGGTGATGACCGGCGCTGC
GGGTATGCCCGTCGCCGTGCTCAATCACAACCGCGTGATGGGTTACATGGTGCCGGCCGAACTGTACGAGGCCATGATGG
AACGCTTGGAAGATCTCGAATTGGGCGAACTGGCCAAGTCGAGGTTGGGTGAAAAAGGCGTACCGGTATCCCTGGATGAC
CTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330WAT7

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References