Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4379053..4379639 Replicon chromosome
Accession NZ_CP063904
Organism Klebsiella pneumoniae strain BM334-2

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag ISA84_RS21170 Protein ID WP_002920800.1
Coordinates 4379271..4379639 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag ISA84_RS21165 Protein ID WP_004174006.1
Coordinates 4379053..4379274 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA84_RS21145 4375210..4376136 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
ISA84_RS21150 4376133..4377410 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
ISA84_RS21155 4377407..4378174 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
ISA84_RS21160 4378176..4378889 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
ISA84_RS21165 4379053..4379274 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ISA84_RS21170 4379271..4379639 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ISA84_RS21175 4379912..4381228 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
ISA84_RS21180 4381335..4382222 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
ISA84_RS21185 4382219..4383064 + 846 WP_004174009.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
ISA84_RS21190 4383066..4384136 + 1071 WP_004213396.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4376133..4384873 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T179333 WP_002920800.1 NZ_CP063904:4379271-4379639 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T179333 NZ_CP084703:496374-496724 [Bacillus shivajii]
TTGATTGTAAAACGTGGTGATGTTTATTTTGCTGACCTTTCACCCGTTGTTGGTTCTGAACAAGGTGGAATCCGACCGGT
TCTTATTATTCAAAACGATATCGGGAACCGCTTTAGCCCGACGGTTATTGTGGCAGCAATTACAGCGCAAATTCAAAAAG
CGAAATTGCCAACACATGTGGAAATTAATGCGAAAAGATATGGATTTGATCGAGATTCCGTCATACTTCTAGAACAAGTC
CGCACCATTGATAAGCAACGTCTAACGGATAAGATCACGCATCTGGATGATGATATGATGGCAAAAGTTAATGATGCTTT
GATGATCAGTCTTGGTTTGATTGACTTTTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT179333 WP_004174006.1 NZ_CP063904:4379053-4379274 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT179333 NZ_CP084703:496091-496369 [Bacillus shivajii]
GTGTCCGAAGAGAACACAAAAAAAATTATGGTAAGCTTACCGCAACATTTAGTGAACGAACTTGATGGTTTACTGGAAAG
ACAGAATGTTAACCGCAGTGAAATAATTCACCAAGCTACGAAAATGTACTTGAGAGAACAAAAGAAACAACAAATCCGCG
AAACGATGCAACAAGGCTATATGGAAATGGCGAAAATTAACTTGAACATTGCAACAGAATCCTTTCTTGTTGAGGAGGAG
GCCGAGAATACGTTGGATCGCTTAGTTAGTGGGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References