Detailed information of TA system TA179270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1699908..1700505
• Replicon: chromosome
• Accession: NZ_CP063898
• Organism: Klebsiella variicola strain BS326-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: ISA87_RS07970
• Protein ID: WP_012542526.1
• Coordinates: 1699908..1700225 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISA87_RS07975
• Protein ID: WP_012542525.1
• Coordinates: 1700218..1700505 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA87_RS07955	1695749..1697176	-	1428	WP_038422225.1	MFS transporter	-
ISA87_RS07960	1697285..1698151	+	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
ISA87_RS07965	1698680..1699723	+	1044	WP_016160437.1	DUF2157 domain-containing protein	-
ISA87_RS07970	1699908..1700225	+	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISA87_RS07975	1700218..1700505	+	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
ISA87_RS07980	1700985..1701638	-	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
ISA87_RS07985	1701758..1702126	-	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
ISA87_RS07990	1702126..1702707	-	582	WP_046881940.1	TetR/AcrR family transcriptional regulator	-
ISA87_RS07995	1702887..1704002	+	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
ISA87_RS08000	1704033..1704374	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
ISA87_RS08005	1704391..1704639	-	249	WP_008805539.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12092.35 Da
• Isoelectric Point: 11.2767

>T179270 WP_012542526.1 NZ_CP063898:1699908-1700225 [Klebsiella variicola]
IFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T179270 NZ_CP084655:c2582022-2581714 [Pectobacterium brasiliense]
ATGCAATATAAAGTTTACCGTAATAACGGTAACAGCAACTCATACCCTTACCTGCTCAATGTCCAAAGTGACATCATTGG
GGAATTACATACCCGCCAGGTGATACCTTTGTTTCCACTACACAACATTGCCAGACCACCAGCGCGAAGACTAACGCCGA
TAGTCACTGTCGAAGGTAACGATTACCTGATAATGACCCATGAAATGGCAAGCGTACGCCGCTCACAACTTGGTCATGAA
GTGATGGACGCGCAGATGTACCGAAAAACAATCAAAGACGCCGTTGATTTTCTGCTTGATGGGTTCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT179270 WP_012542525.1 NZ_CP063898:1700218-1700505 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT179270 NZ_CP084655:c2582264-2582025 [Pectobacterium brasiliense]
ATGCCCGTAACAAGCGTTCGTCACAAAAAAACGGTCAGTGTGACGTTGGAACCCATGCTGCTTCAACAGGCACGTGAAGC
CGGAATTAACCTATCCGCAATACTCACCGAAGCGTTGAAGAGAGAATTTCGCCTGTCTGAAGGGGAAAAATGGAAACAGC
AGAACCAGAAGGCATTACAGGAACTCAACCGTATCACAGATAAACACGGCTTATTATCCGACGATGACAGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2